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RAPID (Rapid Autofocus via Pupil-split Image phase Detection)

By Ludovico Silvestri

Firenze,Toscana,IT
Categories : Area : Computin... Area : Medical ... Area : Neurosci... Technology Read...

Evaluation

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A universal solution for real-time, image-based autofocus in microscopy

We devised a new method for focus stabilization in optical microscopy. Standard methods rely either on contrast optimization (thus working offline) or on triangulation (requiring a reflective surface).

RAPID (Rapid Autofocus via Pupil-split Image phase Detection) extends to the entire field of view of the instrument the phase-detection principle, which is limited to selected points of interest in professional photography.

This method allows direct evaluation of the defocusing state of the system simply using the detected image. Therefore, RAPID is completely agnostic with respect to sample or microscope, and can be applied to all systems, in all settings.

Rapid optichal scheme



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